Perform direct one-step cloning of PCR products with 3'-dA overhangs generated by Taq DNA polymerase and other thermostable DNA polymerases which lack proofreading activity.
An advanced positive-selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Any blunt or sticky-end DNA fragment can be cloned.
Use for synthesis of first-strand cDNA templates from total RNA or polyadenylated RNA using a primer of choice. Cytiva Life Sciences™ Ready-to-Go™ You-Prime First-Strand Beads are perfect for RT-PCR applications.
Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection system.
Cloning of blunt-end or 3'-dA tailed PCR products up to 10kb; Cloning of DNA fragments generated by restriction enzymes; Sequencing of cloned DNA; In vitro and in vivo transcription of cloned inserts from the T7 promoter