Invitrogen™ RNase Cocktail™ Enzyme Mix

Description

RNase Cocktail™ is mixture of two highly purified ribonucleases, RNase A (500U/mL) and RNase T1 (20,000U/mL) and is free of DNase and nicking activities. Digestion of RNA with RNase A alone leaves fragments of RNA which are large enough to be visible on agarose gels and precipitate in ethanol. RNase A cuts after C and U residues, and RNase T1 cuts after G residues. Consequently, mixture of both enzymes results in reduction in RNA fragment size over use of either alone.

• Use RNase Cocktail for all situations, desirable to degrade RNA, i.e. plasmid minipreps and ribonuclease protection assays
• RNase Cocktail is supplied in 50% glycerol for maximum convenience
• Unit concentration: RNase A at 500U/mL and RNase T1 at 20,000U/mL
• Quality Control: RNase Cocktail Enzyme Mix is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and protease activity; functionality is determined in ribonuclease protection assay
• Unit Definitions: RNase A: One unit of RNase A is amount required to give increase in absorption at 286nm of 0.0146 absorbance units per minute in a 1mL volume
• Unit assay conditions: 100mM Tris-acetate (pH 6.5), 1mM EDTA and 1mM cyclic 2', 3'-CMP
• RNase T1: 100 Units of RNase T1 is amount of enzyme that yields an increase in absorption at 260nm of 0.01428 units per min. at room temperature using 60μg/mL yeast total RNA as a substrate

DNA & RNA Purification & Analysis, DNA Extraction, Maxiprep, Midiprep, Miniprep, Nuclease Protection Assays, Nucleic Acid Gel Electrophoresis & Blotting, Plasmid DNA Purification

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