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Invitrogen™ TRIzol™ Max™ Bacterial RNA Isolation Kit
The TRIzol™ Max™ Bacterial RNA Isolation Kit provides a simple and reliable method to improve the isolation of intact total RNA from gram-positive and gram-negative bacteria.
Brand: Invitrogen™ 16096040
640.90 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.
This item is not returnable.
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Description
The TRIzol™ Max™ Bacterial RNA Isolation Kit provides a simple and reliable method to improve the isolation of intact total RNA from gram-positive and gram-negative bacteria. The kit utilizes both the Max™ Bacterial Enhancement Reagent and TRIzol™ Reagent to inactivate endogenous RNases and promote protein denaturing, improving RNA quality and integrity. Isolation of RNA takes about an hour.
Key Features of the TRIzol™ Max™ Bacterial RNA Isolation Kit:
• Formulated for the isolation of RNA from both Gram-positive and Gram-negative bacteria
• Uses two technologies for maximum RNA yield and purity
• Minimizes time required for RNA isolation by eliminating enzymatic and mechanical lytic steps
Two Products in One Kit
The TRIzol™ Max™ Bacterial RNA Isolation Kit combines Max™ Bacterial Enhancement Reagent with TRIzol™ Reagent. A 5-minute pre-treatment of bacteria with Max™ Bacterial Enhancement Reagent, containing chelating agents, detergent and buffer, denatures bacterial proteins and effectively deactivates endogenous RNases. Subsequent bacterial lysis by TRIzol™ Reagent generates a high-quality, minimally degraded RNA product.
Purified RNA is Ideal for Use with a Variety of Applications and Products
Bacterial RNA isolated using the TRIzol™ Max™ Bacterial RNA Isolation Kit is suitable for downstream applications such as real-time PCR (qPCR), RT-PCR, northern blotting, nuclease protection assays, cDNA synthesis, microarray analysis, and dot blot hybridization.
For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
Specifications
| • 2 x 20 mL Max Bacterial Enhancement Reagent; room temperature • 200 mL TRIzol Reagent; room temperature |
|
| 1 hr. | |
| Bacteria (Gram +/-) | |
| Not High-throughput Compatible (Manual) | |
| 200 Preps | |
| Cells: ≤108 Bacterial culture: 1.5 mL |
| Organic Extraction | |
| 50 μL | |
| Total RNA | |
| RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling | |
| Room Temperature | |
| Gram-negative bacteria: >20 μg Gram-positive bacteria: ≤3 μg |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- TRIzol Max Bacterial RNA Isolation Kit
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 1
- Skin corrosion/irritation Category 1 B
Hazard Statement
- H302-Harmful if swallowed.
- H312-Harmful in contact with skin.
- H314-Causes severe skin burns and eye damage.
- H318-Causes serious eye damage.
- H332-Harmful if inhaled.
Precautionary Statement
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P270-Do not eat, drink or smoke when using this product.
- P271-Use only outdoors or in a well-ventilated area.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Thiocyanic acid, compound with guanidine, Phenol, Ammonium thiocyanate
For Research Use Only. Not for use in diagnostic procedures.