Invitrogen™ BacMam Histone H3 Reagent

Description

The BacMam Histone H3 Reagent provides a convenient genetic delivery tool for the expression of GFP-Histone H3 fusion protein in your cell background of interest. The GFP fusion enables sensitive detection of post-translational modifications using LanthaScreen™ TR-FRET Cellular Assay technology.

The BacMam Histone H3 Reagent Lets You:

• Use your cell background of choice with the portability of BacMam
• Conserve precious cell samples with the miniaturizable homogenous assay format
• Identify more relevant inhibitors since the methyl transferases and demethylases are in their natural protein complexes
• Improve data quality with the advantages of TR-FRET

Get More Physiologically Relevant Results

The BacMam Histone H3 allows the investigation of post-translational modifications of histone H3 in your choice of cellular backgrounds, including primary cells. This enables screening for potential inhibitors of histone-modifying enzymes such as kinases, acetylases, deacetylases, methyl transferases, and demethylases in their natural complexes in a physiologically relevant cell type.

BacMam and LanthaScreen™ Convenience Saves Sample and Time

When used with a LanthaScreen™ Tb-anti-Histone H3 antibody against a specific modification, assays can be run in a fully homogenous, addition-only format without any of the washing, lysate transfer, or separation procedures required for traditional methods such as Western blotting and ELISA. By using the same BacMam reagent for GFP-Histone H3, a variety of histone H3 modifications (Ph, Me, Ac) can be monitored just by exchanging the Terbium (Tb) labeled antibody. In addition, the application of the LanthaScreen™ technology includes all of the advantages of TR-FRET detection, including reduced data noise, less interference from fluorescent compounds, and high sensitivity, allowing the use of fewer cells.