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BD Pharmingen™ Transcription-Factor Buffer Set
Optimized for fixing and permeabilizing cells prior to immunofluorescent staining and flow cytometric analysis of cells that express specific intracytoplasmic and intranuclear proteins
Brand: BD 562574
Alert:
5x Perm/Wash Buffer contains ≤0.09% sodium azide. Sodium azide yields highly toxic hydrazoic azid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. 4x Fix/Perm Buffer contains diethylene glycol and formaldehyde and is harmful.
Includes: Fix/Perm Buffer (4X), Diluent Buffer, Perm/Wash Buffer (5X)
Description
This set is designed to minimize processing time, to reduce nonspecific staining, to increase resolution of positively stained cells and to significantly reduce cell loss during fixation, permeabilization and staining procedures. This is useful for flow cytometric
detection of many proteins. It supports multiwell-plate high-throughput and bulk sample analyses and applications that require overnight sample storage.
- High sensitivity for many transcription factors including FoxP3, RORgt, Bcl-6, and T-bet
- Compatible with many cell types, cell surface staining, and tandem dyes
- Size: 100 tests
Intracellular staining (flow cytometry)
Specifications
| Flow cytometry | |
| Fix/Perm Buffer (4X), Diluent Buffer, Perm/Wash™ Buffer (5X) | |
| 100 Tests |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- BD Pharmingen Transcription Factor Buffer Set
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 4
- Carcinogenicity Category 1B
- Serious eye damage/eye irritation Category 2
- Germ cell mutagenicity Category 2
- Skin corrosion/irritation Category 2
- Skin sensitiser Category 1
- Specific target organ toxicity Category 3
Hazard Statement
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H317-May cause an allergic skin reaction.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
- H341-Suspected of causing genetic defects.
- H350-May cause cancer.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash thoroughly after handling.
- P272-Contaminated work clothing should not be allowed out of the workplace.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P312-Call a POISON CENTER/doctor/if you feel unwell.
- P333+P313-If skin irritation or rash occurs: Get medical advice/attention.
- P337+P313-If eye irritation persists: Get medical advice/attention.
Supplemental information
- MIXTURE LIST-Contains : formaldehyde, methanol