Invitrogen™ Bovine anti-Chicken IgY Secondary Antibody, Agarose

Description

SA1-9588 contains 1 mL of antibody linked to agarose beads (50%) in PBS with 0.02% sodium azide. This antibody reacts with the heavy and light chains of the immunoglobulin molecule. SA1-9588 immunogen is immunoglobulin isolated from chicken serum. To obtain optimal immunoprecipitation, incubate the beads with samples containing IgY antibodies-protein complex for at least 10 minutes. Binding capacity of the beads is approximately 450 μg to 500 μg IgY per mL solid beads.

Anti-Chicken secondary antibodies are affinity-purified antibodies with well-characterized specificity for the chicken immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.