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IBA Lifesciences CD3 Fab-TACS™ Gravity Kit
IBA Lifesciences™ CD3 Fab-TACS™ Gravity Kit is for reversible cell isolation delivering label-free target cells.
Brand: IBA Lifesciences 6-3201-010
This item is not returnable.
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Description
- The kit allows the selection of CD3+ T cells directly from whole blood.
- CD3 Fab-Strep, human
- Buffer CI (10x)
- Biotin stock solution
- Fab-TACS™ Gravity Adapter
- Fab-TACS™ Flow Restrictor
- The Fab-TACS™ Gravity column is filled with a Strep-Tactin™ coated matrix made of cell-grade agarose.
- Strep-tagged Fab fragments (Fab-Streps) specifically bind to the matrix.
- Subsequently, whole blood or other blood preparations pass through the column.
- Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to the target cell.
- Non-target cells are efficiently washed away.
- In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps.
- After elution, the Fab-Streps self-dissociate from the cell surfaces.
- The label-free authentic target cells are now ready for further downstream applications.
Specifications
| Isolation of label-free cells | |
| 2°C to 8°C | |
| 150 mL Whole Blood |
| Non-magnetic Cell Isolation Kit | |
| Human Whole Blood | |
| 10 Columns, Human |