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Corning™ DMEM (Dulbecco's Modified Eagle's Medium)

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Quantity:
6 x 500 mL
6 x 1 L
1 x 10 L
1 x 20 L
With Additives:
Glucose
Glucose, Glutamine
Glucose, Glutamine, Sodium Pyruvate
Glucose, Sodium Pyruvate
Glucose, Sodium Pyruvate, HEPES
Glutamine, Glucose
Without Additives:
No Glutamine
No Glutamine, No Phenol Red
No Glutamine, No Sodium Pyruvate
No Sodium Pyruvate
None
Product Type:
DMEM Media, 1 g/L Glucose
DMEM Media, 4.5 g/L Glucose
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10 L
20 L
Pack of 6
Beschreibung
- Substitution of Pyridoxine HCl for Pyridoxal ensures improved stability over the original formulation
- DMEM is a modification of Basal Medium Eagle (BME) containing four-fold concentrations of the amino acids and vitamins found in BME
- Original formulation was used to culture embryonic mouse cells and has since been modified to support variety of normal and transformed cells, such as primary cultures of mouse and chicken cells
- Formulations can differ in amount or absence of components such as glucose, sodium pyruvate, sodium bicarbonate and L-glutamine
- Tested to determine pH and osmolality
- Low levels of endotoxin, and absence of contamination
- Tested for bacterial endotoxin using the Limulus Amoebocyte Lysate (LAL) Chromogenic Assay
- Osmolality: 335±mOsm/kg H2O
- Mycoplasma: Tested Negative
- Sterility: Pass
Spezifikation
Spezifikation
| Concentration | 1 X |
| Form | Liquid |
| Product Type | DMEM Media, 4.5 g/L Glucose |
| With Additives | Glucose, Sodium Pyruvate |
| Without Additives | No Glutamine, No Phenol Red |
| For Use With (Application) | To assess growth promotion and cytotoxicity characteristics |
| Quantity | 6 x 500 mL |
| Endotoxin Level | <0.25EU/mL |
| Formulation | Inorganic Salts: 200mg/L CaCl2 (anhydrous), 0.1mg/L Fe(NO3)3.9H2O, 400mg/L KCl, 97.7mg/L MgSO4 (anhydrous), 6400mg/L NaCl, 125mg/L NaH2PO4.H2O, 3700mg/L NaHCO3; Amino Acids: 84mg/L L-Arginine. HCl, 62.57mg/L L-Cystine.2HCl, 30mg/L Glycine, 42mg/L L-Histidine.HCl.H2O, 104.8mg/L L-Isoleucine, 104.8mg/L L-Leucine, 146.2mg/L L-Lysine. HCl, 30mg/L L-Methionine, 66mg/L L-Phenylalanine, 42mg/L L-Serine, 95.2mg/L L-Threonine, 16mg/L L-Tryptophan, 103.79mg/L L-Tyrosine.2Na.2H2O, 20mg/L L-Valine; Vitamins: 4mg/L D-Calcium pantothenate, 4mg/L Choline chloride, 4mg/L Folic Acid, 7.2mg/L i-Inositol, 4mg/L Nicotinamide, 4mg/L Pyridoxine. HCl, 0.4mg/L Riboflavin, 4mg/L Thiamine. HCl; Other: 4500mg/L D-Glucose, 110mg/L Sodium pyruvate; Added: 584mg/L L-glutamine Powder, 20 mL/L 200 mM Solution. |
| Content And Storage | 2 to 8°C |
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