Invitrogen™ Collibri™ PCR-free PS DNA Library Prep Kits for Illumina Systems

Description

The Collibri PCR-free PS DNA Library Preparation Kit offers higher confidence in SNP and INDEL calling due to better coverage of challenging genomic regions from inputs as low as 500 ng of physically sheared double-stranded DNA, cfDNA, or amplicons (≤300 bp) of any species. The entire library preparation protocol takes about three hours with a PCR workflow. Additionally, a PCR-free version is available for inputs starting at 500 ng DNA.

The Collibri PCR-free PS DNA Library Preparation Kit offers higher confidence in SNP and INDEL calling due to better coverage of challenging genomic regions from inputs as low as 500 ng of physically sheared double-stranded DNA, cfDNA, or amplicons (≤300 bp) of any species. The entire library preparation protocol takes about three hours with a PCR workflow. Additionally, a PCR-free version is available for inputs starting at 500 ng DNA.

The Collibri PCR-free PS DNA Library Preparation Kit:
• Includes all necessary components in a single kit* for quick use
• Is empowered on miniaturization liquid handlers to cut on costs
• Provides in-process visual feedback for easier handling
• Is suitable for conventional automation to increase throughput

* The kit consists of enzymatic modification, size selection/clean-up, and indexed adapter-containing modules, except for the Core kit, which enables Collibri combination with desired indexed adapters.

The Collibri PCR-free PS DNA Library Preparation Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step. The kit generates a consistently high yield of library from any amount of input DNA (including the PCR-free version), which is enough for library quantification and sequencing on Illumina instruments.

Applications
The Collibri PCR-free PS DNA Library Preparation Kit is recommended for:
• Whole genome sequencing
• Resequencing
• De novo assembly