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Cytiva Con A Sepharose™ 4B Medium

An affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids

Brand: Cytiva 17-0440-01

Product Code. 11524905

This item is not returnable. View return policy

Description

Highly suited for isolation of cell surface glycoproteins from detergent-solubilized membranes.

Con A coupled to Sepharose, in a 4% agarose matrix via cyanogen bromide activation
Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
Group-specific adsorbent for molecules containing sugars
Concentration of immobilized Con A, approx. 13mg/mL medium; binding capacity for porcine thyroglobulin, approx. 30mg/mL medium
Stable in the pH range 4 to 9, and in the presence of all commonly used buffers
Average particle size: 90μm
Delivered in 0.1M acetate buffer, pH 6.0, containing 1M NaCl, 1mM CaCl₂, 1mM MgCl₂, 1mM MnCl₂, and 20% ethanol

Specifications

Ligand Concentration	13mg Con A/mL Drained Medium
Particle Size	90 μm
Quantity	100 mL
For Use With (Application)	For separation and purification of Glycoproteins, Polysaccharides and Glycolipids, isolates cell surface Glycoproteins from detergent-solubilized membranes

Product Suggestions Safety and Handling

Safety and Handling

Product Identifier

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Precautionary Statement

P280-Wear protective gloves/protective clothing/eye protection/face protection.
P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P241-Use explosion-proof electrical/ventilating/lighting/and all material-handling/ equipment.
P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
P235-Keep cool.
P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information