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MP Biomedicals™ SurePRIME DNA Polymerase
SurePRIME DNA Polymerase is a thermostable DNA polymerase that is suitable for all PCR applications requiring hot start conditions.
Brand: MP Biomedicals™ 11EPHSP025
This item is not returnable.
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Description
- SurePRIME DNA Polymerase is an improved Hot Start DNA Polymerase used to increase specificity and product yield.
- SurePRIME DNA Polymerase is heat-activated after a pre-incubation step at 95°C and is then functionally equivalent to classical Taq DNA Polymerase.
- Chemically modified recombinant Taq DNA polymerase.
- The enzyme is a highly purified form of recombinant Taq DNA Polymerase that has been chemically modified by the addition of heat-labile blocking groups to specific amino acid residues. Prior to PCR, in its inactive state, SurePRIME DNA Polymerase is incapable of extending primer-dimers or mis-annealed primer-template species that form below the specific annealing temperature. The 95°C incubation step therefore serves to activate the enzyme and to ensure a completely clean initial PCR cycle.
- Easy to use.
- Once activated SurePRIME DNA Polymerase is functionally equivalent to Taq DNA Polymerase and so the only PCR program modification required is the addition of a 15 min. pre-incubation step at 95°C. Efficiency can be improved by a progressive activation (initial activation 5 min. at 95°C followed by 1 min. activation at 95°C at each cycle).
- Save time and effort.
- Switching to SurePRIME DNA Polymerase avoids wasting time with complicated and time-consuming manual Hot Start procedures or less efficient antibody-binding solutions.
Specifications
| Store at -20°C. | |
| Chemically modified recombinant Taq DNA polymerase | |
| Heat Activated Polymerase |