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Invitrogen™ Dynabeads™ mRNA DIRECT™ Purification Kit
The Dynabeads™ mRNA DIRECT™ Kit is designed for simple and rapid isolation of pure, intact polyadenylated (polyA) mRNA directly from the crude lysate of animal and plant cells and tissues.
Brand: Invitrogen™ 61012
791.00 EUR valid until 2024-11-30
Use promo code "21651" to get your promotional price.
Includes: 10mL Dynabeads oligo (dT)25, 60mL Lysis/Binding Buffer, 120mL Washing Buffer A, 60mL Washing Buffer B, 15mL 10mM Tris-HCl (Elution buffer), Contains enough reagents for 40 standard isolations.
Description
The Dynabeads™ mRNA DIRECT™ Kit is designed for simple and rapid isolation of pure, intact polyadenylated (polyA) mRNA directly from the crude lysate of animal and plant cells and tissues. The isolated mRNA is suitable for use in all downstream applications.
Advantages of the Dynabeads mRNA DIRECT Kit:
• Fast—15 minute procedure yields pure, intact mRNA
• Highly pure mRNA isolation—best choice upstream of cDNA synthesis
• Sensitive mRNA isolation—enables cDNA synthesis and cDNA library construction from ultra-small starting samples (enables cDNA library construction from a single cell)
System overview
The isolation protocol relies on base pairing between the polyA residues at the 3' end of most mRNA, and the oligo (dT)25 residues covalently coupled to the surface of the Dynabeads. Other RNA species lacking a polyA tail will not hybridize to the beads and are readily washed away. Ribosomal RNA, DNA, proteins, and small RNA molecules (such as transfer RNA, micro RNA, and small nucleolar RNA) do not bind to the beads and are discarded. RNase inhibiting agents in the Lysis/Binding Buffer together with stringent hybridization and washing conditions ensure the isolation of pure, intact mRNA from crude samples rich in RNase, without the use of strong chaotropic agents. The mRNA purification beads specifically target and capture the mRNA transcriptome from an extremely wide variety of crude starting samples (see protocol). 1 mg of Dynabeads Oligo (dT)25 beads (200 μL) binds up to 2 μg of mRNA. A typical mammalian cell contains about 10–30 pg of total RNA, from which 1–5% is mRNA.
Order Info
Shipping Condition: Room Temperature
Specifications
• 10 mL Dynabeads Oligo (dt)25, 4°C |
|
| 10 to 100 μL | |
| mRNA | |
| Automated Liquid Handling Systems | |
| Beads may be reused for multiple extractions | |
| Room Temperature | |
| 2 μg mRNA per 200 μL of beads (Binding capacity) |
| Magnetic Bead | |
| Cells, Plants, Plasma, Serum, Tissue, Total RNA, Blood | |
| High-throughput Compatible | |
| RT-PCR, qPCR, cDNA library construction, microarray analysis | |
| 10 mL | |
| Cells: ≤106 Plant: ≤400 mg Tissue: ≤200 mg |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- Dynabeads mRNA DIRECT Purification Kit
Signal Word
- Warning
Hazard Category
- Serious eye damage/eye irritation Category 2
Hazard Statement
- H319-Causes serious eye irritation.
Precautionary Statement
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P337+P313-If eye irritation persists: Get medical advice/attention.
Supplemental information
- MIXTURE LIST-Contains : Lithium chloride
For Research Use Only. Not for use in diagnostic procedures.