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  5. IBA Lifesciences pASG-IBA E. coli Expression Vector with No Secretion

IBA Lifesciences pASG-IBA E. coli Expression Vector with No Secretion
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Product Code. 15653748 Shop All IBA Lifesciences Products
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Vector:
pASG-IBA103
pASG-IBA105
pASG-IBA123
pASG-IBA143
pASG-IBA145
pASG-IBA162
pASG-IBA164
pASG-IBA167
pASG-IBA168
pASG-IBA23
pASG-IBA3
pASG-IBA43
pASG-IBA45
pASG-IBA5
pASG-IBA62
pASG-IBA64
pASG-IBAwt1
Pakningsstørrelse:
5µg
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Artikelnummer. 15653748

Brand: IBA Lifesciences 54123001

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Beskrivelse

Regulate the expression of proteins with a vector that carries the inducible tetracycline promoter/operator. IBA Lifesciences GmbH E. coli Expression Vector with No Secretion allows for the expression of proteins in E.coli.

  • The pASG-IBA vectors are designed for expression of target proteins without affinity tag in E. coli.
  • The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins.
  • The vectors can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli.
  • Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity.
Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Tekniske data

Inducing Agent Inducible with anhydrotetracycline
Marker Gene Ampicillin
Promoter Tet
Product Type Vector
Content And Storage Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
Species E.coli
Protein Tag Twin-Strep-tag, GST Tag
Cloning Method Direct Cloning
Concentration 250 ng/μL
Primer Forward
Quantity 5 μg
Vector pASG-IBA123
For Use With (Application) Vector for recombinant expression in E. coli
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