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Gibco™ ExpiFectamine™ 293 Transfection Kit
Designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells.
Brand: Gibco™ A14524
935.78 EUR valid until 2024-11-30
Use promo code "21651" to get your promotional price.
This item is not returnable.
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Description
The ExpiFectamine™ 293 Transfection Kit is a core component of the Expi293™ Expression System. It is designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells. The high-efficiency, cationic, lipid-based transfection reagent and transfection enhancers are designed to power the highest possible level of protein expression from Expi293F™ cells cultured in Expi293™ Expression Medium
- Designed specifically for transfection of high density suspension cell culture, with matching transfection enhancers that boost transfection performance and protein expression
- Achieves protein yields 2- to 10-fold higher than other transfection reagents used on high density 293 cell cultures
- Employs the same transient expression protocols typically used in current low-density 293 suspension culture systems to easily switch from low density systems to the high yield, high density Expi293™ Expression System
- Provides robust and reproducible transfection results, giving users greater confidence in their results
- Scale transfections for culture volumes of less than 1 mL to greater than 10 liters, while maintaining equivalent volumetric protein yields
The ExpiFectamine™ 293 Transfection Kit is specifically designed to work with Expi293F™ cells in the Expi293™ Expression System. The transfection kit consists of the cationic lipid-based ExpiFectamine™ 293 Reagent and ExpiFectamine™ 293 Transfection Enhancers 1 and 2. The enhancers are added 18 to 22 hours post-transfection to boost transfection, cell viability, and protein expression. The protocol for use of ExpiFectamine™ 293 Transfection components is very similar to transfection protocols for other transient expression reagents, such as 293fectin™ and FreeStyle™ MAX.
Specifications
| Expi293F™ | |
| 2.7 mL (3 x 0.9 mL) ExpiFectamine™ 293 Reagent 5 mL ExpiFectamine™ 293 Transfection Enhancer 1 50 mL ExpiFectamine™ 293 Transfection Enhancer 2 Sufficient for transfection of 1 L of culture Store at 2°C to 8°C |
|
| Plasmid DNA | |
| Transfection | |
| Transfection Kit | |
| For 1 L of culture |
| Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free | |
| Established Cell Lines | |
| Lipid-based Transfection | |
| ExpiFectamine™ | |
| Cell Culture Grade | |
| No |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- ExpiFectamine 293 Transfection Kit
Signal Word
- Warning
Hazard Category
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
Hazard Statement
- H302-Harmful if swallowed.
- H312-Harmful in contact with skin.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H335-May cause respiratory irritation.
Precautionary Statement
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P312-IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Supplemental information
- MIXTURE LIST-Contains : 3-Fluoro-4-nitrophenol
For Research Use Only. Not for use in diagnostic procedures.