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Thermo Scientific™ EZ-Link™ NHS-PEG12-Biotin, No-Weigh™ Format
Biotinylate protein amines and maximize solubility of proteins with this very long (56.0A) NHS-ester biotinylation reagent containing a pegylated, water-soluble spacer arm.
Brand: Thermo Scientific™ A35389
This item is not returnable.
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Description
Thermo Scientific™ EZ-Link NHS-PEG12-Biotin is very long, pegylated biotinylation reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.
Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging helps prevent the loss of reagent reactivity and contamination over time by minimizing the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.
- Protein labeling: Biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
- Amine-reactive: Reacts with primary amines (-NH2), such as side-chain of lysines (K) or amino-termini of polypeptides
- PEGylated: Spacer arm contains hydrophilic, 12-unit, polyethylene glycol (PEG) group
- Enhances solubility: PEGylation imparts water solubility to biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
- Irreversible: Forms permanent amide bonds; spacer arm cannot be cleaved
- Very long reach: Spacer arm (total length added to target) is 56A0; this reduces steric hindrance when binding to avidin molecules
Specifications
| 10 x 1 mg | |
| Room Temperature | |
| Water | |
| Biotin & Analogs |
| Store desiccated at -5 to -30°C. | |
| EZ-Link™, No-Weigh™ | |
| Solid |
For Research Use Only. Not for use in diagnostic procedures.