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Thermo Scientific™ BseGI (BtsCI) (10 U/μL)
Cut at GGATG(2/0)^ sites with BseGI (BtsCI) restriction enzyme, which performs best at 55°C in Tango buffer (Isoschizomers: BstF5I, BtsCI, FokI).
Brand: Thermo Scientific™ ER0871
This item is not returnable.
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Description
Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
5'...G G A T G N N▵...3'
3'...C C T A C▵N N ...5'
Conditions for 100% Activity:
- 1X Buffer Tango™: 33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
- Incubate at 55°C
Storage Buffer:
- BseGI is supplied in:10mM Tris-HCl (pH7.4 at 25°C), 100mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol
Ligation and Recleavage:
- After 50-fold overdigestion with BseGI, more than 95% of the DNA fragments can be ligated and recut
Methylation Effects:
- Dam: never overlaps — no effect
- Dcm: may overlap — no effect
- CpG: may overlap — no effect
- EcoKI: never overlaps — no effect
- EcoBI: may overlap — effect not determined
Note:
Incubation at 37°C results in 25% activity.
Specifications
| 10 U/μL | |
| BseGI (BtsCI) | |
| Yes | |
| Yes | |
| Restriction Enzyme |
| Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive | |
| 10X Buffer Tango | |
| 55°C | |
| 500 U | |
| Scarless Cloning |
For Research Use Only. Not for use in diagnostic procedures.