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  5. Thermo Scientific™ CpoI (RsrII) (10 U/μL)
  6. 10528160

Thermo Scientific™ CpoI (RsrII) (10 U/μL)

The CpoI (RsrII) restriction enzyme recognizes CG^GWCCG sites and cuts best at 37°C in Tango buffer (Isoschizomers: CspI, Rsr2I, RsrII).

Brand:  Thermo Scientific™ ER0742

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Product Code. 10528160

  • 401.00 € / 1000 units

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Description

Description

Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...C G▵G W C C G...3'

3'...G C C W G▵G C...5'

Conditions for 100% Activity:

  • 1X Buffer Tango:33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • CpoI is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with CpoI, more than 95% of the DNA fragments can be ligated and recut

Methylation Effects:

  • Dam: never overlaps-no effect
  • Dcm: never overlaps-no effect
  • CpG: completely overlaps-blocked
  • EcoKI: never overlaps-no effect
  • EcoBI: never overlaps-no effect

Compatible Ends:

  • Cfr13I, Eco47I, Psp5II, SanDI
TRUSTED_SUSTAINABILITY
Specifications

Specifications

10 U/μL
CpoI (RsrII)
Yes
No
Restriction Enzyme
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
10X Buffer Tango
37°C
1,000 units
Traditional Cloning
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