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Thermo Scientific™ NotI, HC (50 U/μL)
Thermo Scientific™ Fermentas NotI is available as a FastDigest enzyme which is used for rapid DNA digestion.
Brand: Thermo Scientific™ ER0593
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Description
Thermo Scientific™ NotI is available as a FastDigest enzyme which is used for rapid DNA digestion, more than 95% of the digested DNA fragments can be ligated and recut at a 5'-termini concentration of 0.5μM after a 50-fold overdigestion (3U/μg DNA x 17 hours) with NotI.
5'..G C▵G G C C G C...3'
3'..C G C C G G▵C G...5'
Conditions for 100% Activity:
- 1X Buffer O: 50mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2, 100mM NaCl and 0.1mg/mL BSA
- Incubate at 37°C
Storage Buffer:
- NotI is supplied in: 20mM Tris-HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton X-100, 0.2mg/mL BSA and 50% (v/v) glycerol
Ligation and Recleavage:
- After 50-fold overdigestion with NotI, more than 95% of DNA fragments can be ligated and recut
Methylation Effects:
- Dam: never overlaps —no effect
- Dcm: never overlaps —no effect
- CpG: completely overlaps —blocked
- EcoKI: never overlaps —no effect
- EcoBI: never overlaps —no effect
Digestion of Agarose-embedded DNA:
- Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded Adenovirus-2 DNA in 16 hours
Compatible Ends:
- Bsp120I, CfrI, Eco52I
Note:
Assayed using pTZ19RJL2 plasmid DNA digested with BseLI. Supercoiled plasmids may require up to 5-fold more NotI for complete digestion than linear DNAs.
Specifications
| 50 U/μL | |
| Not I | |
| Yes | |
| No | |
| Restriction Enzyme |
| CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive | |
| 10X Buffer O | |
| 37°C | |
| 1,500 units | |
| Traditional Cloning |
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Product Title
Thermo Scientific™ NotI, HC (50 U/μL) > 50U/μL, HC, 1500U
For Research Use Only. Not for use in diagnostic procedures.
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