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Thermo Scientific™ RiboRuler High Range RNA Ladder
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Description
RiboRuler High Range RNA Ladder 200 to 6000 bases is a mixture of 8 chromatography purified single-stranded RNA transcripts (200 to 6000 bp long), produced from specific templates that contain a fragment of the pTZ19R polylinker and Lambda phage fragments.
The RiboRuler High Range RNA Ladder is available in 1 mM EDTA, pH 6.0.
RiboRuler High Range RNA Ladder is composed of chromatography-purified transcripts that makes them free from NTPs and RNA degradation products. The concentration of each RNA transcript is determined spectrophotometrically (see photo for precise quantities). To minimize the possibility of contamination during the use, the ladder is aliquotted in smaller volumes.
RiboRuler High Range RNA Ladder is optimal for end-labeling using T4 Polynucleotide Kinase (see protocol in the Resources) and is ideal for Northern blots.
Highlights
- Sharp bands of uniform intensity
- Easy-to-remember band sizes and quantities
- Predetermined concentration of ladder bands allows for approximate RNA quantification
- Supplied with 2X RNA Loading Dye
- Stable for 6 months at -20°C
Specifications
Specifications
| Concentration | 2X |
| Gel Type | Agarose |
| Label or Dye | Xylene Cyanol FF, Bromophenol Blue, Ethidium Bromide |
| Product Type | High Range DNA Ladder |
| Size Range | 200 to 6000 bp |
| Product Line | RiboRuler |
| Quantity | 5 x 20 μL |
Safety and Handling
- 2X Loading Dye Solution
- Danger
- Carcinogenicity Category 2
- Reproductive toxicity Category 1B
- Specific target organ toxicity after repeated exposure Category 2
- H351-Suspected of causing cancer.
- H360-May damage fertility or the unborn child.
- H373-May cause damage to organs.
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P314-Get medical advice/attention if you feel unwell.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : formamide
For Research Use Only. Not for use in diagnostic procedures.
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