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Thermo Scientific™ GeneBLAzer™ CCKAR HEK 293T DA Assay Kit
The GeneBLAzer™ CCKAR-NFAT-bla CHO-K1 cells contain the human Cholecystokinin A Receptor (CCKAR) stably integrated into the GeneBLAzer™ NFAT-bla CHO-K1 cell line.
Brand: Thermo Scientific™ K1623
This item is not returnable.
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Description
The GeneBLAzer™ CCKAR-NFAT-bla CHO-K1 cells contain the human Cholecystokinin A Receptor (CCKAR) stably integrated into the GeneBLAzer™ NFAT-bla CHO-K1 cell line. GeneBLAzer™ NFAT-bla CHO-K1 (Cat. No. K1447) contains a beta-lactamase (bla) reporter gene under control of a NFAT response element stably integrated into CHO-K1 cells.
The CCKAR-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations using established ligands. In addition, CCKAR-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
Specifications
| NM_000730.2 | |
| Division-Arrested Cells | |
| Fluorescence | |
| CCKAR | |
| GeneBLAzer™ | |
| BLA (Beta-Lactamase) | |
| Division-Arrested Cells Assay Kit | |
| GeneBLAzer™ |
| Cell-based beta lactamase reporter gene | |
| Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol). Includes: • GeneBLAzer™ CCKAR HEK 293T DA cells • LiveBLAzer™-FRET B/G Loading Kit, 70 μg • Solution D • GeneBLAzer™ CCKAR HEK 293T DA Assay Protocol • LiveBLAzer™-FRET B/G Loading Protocol • Certificate of Analysis The GeneBLAzer™ CCKAR HEK 293T DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
|
| GPCRs | |
| Single Cell Clone Origin | |
| 8 x 106 Cells | |
| CCKAR | |
| 293T (HEK) | |
| GPCR |
For Research Use Only. Not for use in diagnostic procedures.