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Thermo Scientific™ GeneBLAzer™ EDG3-Gα15-NFAT-bla HEK 293T Cells
GeneBLAzer™ EDG3-Gα15-NFAT-bla HEK293T cells contain the human Endothelial Differentiation Receptor 3 (EDG3) stably integrated into the GeneBLAzer™ EDG3-Gα15-NFAT-bla HEK293T cell line.
Brand: Thermo Scientific™ K1713
This item is not returnable.
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Description
GeneBLAzer™ EDG3-Gα15-NFAT-bla HEK293T cells contain the human Endothelial Differentiation Receptor 3 (EDG3) stably integrated into the GeneBLAzer™ EDG3-Gα15-NFAT-bla HEK293T cell line. GeneBLAzer™ Gα15-NFAT-bla HEK293T cells (Cat. No. K1539) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element and the Promiscuous G protein, Gα15.
The GeneBLAzer™ EDG3-Gα15-NFAT-bla HEK293T cells are functionally validated for Z' and EC50 concentrations of S1P. In addition, GeneBLAzer™ EDG3-Gα15-NFAT-bla HEK293T cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.
EDG-3/S1P-3(Sphingosine-1-Phosphate-3) is a Gq/Gi/Go/Gα13/Gα12 coupled GPCR. EDG-3 has been shown to be responsible for bradycardia that has been induced in clinical trials as a side effect of the immunosuppressive drug FTY720 that targets EDG-1. EDG-3 has also been shown to induce vasodilation in response to sphingosylphosphorylcholine (SPC), sphingosine-1-phosphate (S1P), and lysosulfatide (LSF). The EDG-3 Receptor has also been shown to play a cooperative or redundant role in angiogenesis with EDG-1.
For Academic pricing please login or contact us at discoverysciences@invitrogen.com.
Specifications
| NM_005226.2 | |
| Dividing Cells | |
| Fluorescence | |
| EDG3 | |
| Single Cell Clone Origin | |
| 2 x 106 Cells | |
| EDG3 | |
| 293T (HEK) | |
| GPCR |
| Cell-based beta lactamase reporter gene | |
| LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes: • GeneBLAzer™ EDG3-Gα15-NFAT-bla HEK293T cells • GeneBLAzer™ EDG3-Gα15-NFAT-bla HEK293T Assay Protocol • Certificate of Analysis The GeneBLAzer™ EDG3-Gα15-NFAT-bla HEK293T cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
|
| FRET | |
| EDG3-Galpha15-NFAT-bla HEK293T cells, EDG3-Galpha15-NFAT-bla HEK293TAssay Protocol, Certificate of Analysis | |
| GeneBLAzer™ | |
| BLA (Beta-Lactamase) | |
| Dividing Cells | |
| GeneBLAzer™ |
For Research Use Only. Not for use in diagnostic procedures.