Invitrogen™ GeneBLAzer™ EDNRA HEK 293T DA Assay Kit

Description

This cell line is available as dividing cells by requesting a quote for K1223 from dividingcells@invitrogen.com

GeneBLAzer™ EDNRA HEK 293T DA (Division Arrested) cells and EDNRA-NFAT-bla HEK 293T cells contain the human Endothelin type A (EDNRA) receptor stably integrated into the CellSensor™ NFAT-bla HEK 293T cell line. CellSensor™ NFAT-bla HEK 293T cells (Cat. no.K1129) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (NFAT). Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates.

DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both EDNRA HEK 293T DA cells and EDNRA-NFAT-bla HEK 293T cells are functionally validated for Z’-factor and EC₅₀ concentrations of ET-1 (Figure 1). In addition, EDNRA-NFAT-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Endothelin receptor type A (ENDRA) mediates signaling of endothelin proteins that regulate several critical biological processes, including development and function of blood vessels, production of certain hormones, and stimulation of cell growth and division. Defects in EDNRA-mediated signaling have been implicated in a wide range of diseases, including cancer, hypertension, and cardiovascular disorders.


Storage:

GeneBLAzer™ EDNRA HEK 293T DA Cells and EDNRA-NFAT-bla HEK 293T Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.


Contents:
GeneBLAzer™ EDNRA HEK 293T DA Assay Kit
Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol). Includes:

EDNRA HEK 293T DA cells

LiveBLAzer™-FRET B/G Loading Kit, 70μg

Solution D, 1ml

2 protocols

Certificate of Analysis
GeneBLAzer™ EDNRA HEK 293T DA cells
Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:

EDNRA HEK 293T DA cells

1 protocol

Certificate of Analysis
GeneBLAzer™ EDNRA-NFAT-bla HEK 293T cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).Includes:

EDNRA-NFAT-bla HEK 293T cells

1 protocol

Certificate of Analysis