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Thermo Scientific™ GeneBLAzer™ ERβ-UAS-bla GripTite™ Cells
ER beta-UAS-bla GripTite™ 293 cells contain the ligand-binding domain (LBD) of the human Estrogen receptor beta (ER beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla GripTite™ 293 cell line.
Brand: Thermo Scientific™ K1689B
This item is not returnable.
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Description
ER beta-UAS-bla GripTite™ 293 cells contain the ligand-binding domain (LBD) of the human Estrogen receptor beta (ER beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla GripTite™ 293 cell line. GeneBLAzer™UAS-bla GripTite™ 293 cells stably express a betalactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. ER beta-UAS-bla GripTite™293 cells are functionally validated for Z' and EC50 concentrations of 17-beta Estradiol. In addition, ER beta-UAS-bla GripTite™293 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time. Additional testing data using alternate stimuli are also available.
Specifications
| Dividing Cells | |
| Fluorescence, Fluorescent | |
| Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays | |
| GeneBLAzer™ ER beta -UAS-bla GripTite™ cells Includes: ER beta-UAS-bla GripTite™ cells, Protocol, Certificate of Analysis | |
| GeneBLAzer™ | |
| GripTite Cells | |
| BLA (Beta-Lactamase) | |
| Cell Line |
| ER beta-UAS-bla GriptTite™ cells (K1091) Shipping: Dry Ice Storage: -80°C (liquid nitrogen), Liquid nitrogen, room temperature |
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| GeneBLAzer™ ERβ-UAS-bla GripTite™ Cells, 2 x 106 Cells, Fluorescent Detection Method, Nuclear Receptors Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage | |
| Nuclear Receptors | |
| 2 x 106 Cells | |
| 2 x 106 Cells | |
| GripTite™ 293 | |
| Room Temperature |
For Research Use Only. Not for use in diagnostic procedures.