missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Thermo Scientific™ GeneBLAzer™ Gα15-NFAT CHO-K1 DA Assay Kit
The GeneBLAzer™ Gα15-NFAT CHO-K1 DA (Division Arrested) cell line contains the promiscuous G protein, Gα15, stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line.
Brand: Thermo Scientific™ K1453
This item is not returnable.
View return policy
Description
The GeneBLAzer™ Gα15-NFAT CHO-K1 DA (Division Arrested) cell line contains the promiscuous G protein, Gα15, stably integrated into the CellSensor™ NFAT-bla CHO-K1 (Cat. No. K1078) cell line. NFAT-bla CHO-K1 cells contain a beta-lactamase reporter gene under control of an NFAT response element stably integrated into CHO-K1 cells. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
This product can serve as a negative control in screening assays performed with specific G-protein coupled receptor (GPCR) DA cells that were made from this same background. These cells can also be used in other assays where a sensitive readout to intracellular changes in calcium is needed.
Specifications
| Division-Arrested Cells | |
| Fluorescence | |
| Gα15 | |
| 1 Plate | |
| Division-Arrested Cells Assay Kit | |
| GeneBLAzer™ |
| Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol). Includes: • GeneBLAzer™ Gα15-NFAT CHO-K1 DA cells • LiveBLAzer™-FRET B/G Loading Kit, 70 μg • Solution D • GeneBLAzer™ Gα15-NFAT CHO-K1 Assay Protocol • LiveBLAzer™-FRET B/G Loading Protocol • Certificate of Analysis The GeneBLAzer™ Gα15-NFAT CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
|
| GPCRs | |
| GeneBLAzer™ | |
| BLA (Beta-Lactamase) | |
| CHO-K1 | |
| GPCR |
For Research Use Only. Not for use in diagnostic procedures.