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Thermo Scientific™ GeneBLAzer™ HCRTR1-NFAT-bla CHO-K1 Cells
The GeneBLAzer™ HCRTR1-NFAT-bla CHO-K1 cells contain the human Hypocretin (Orexin) Receptor 1 (HCRTR1), stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line.
Brand: Thermo Scientific™ K1446
This item is not returnable.
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Description
The GeneBLAzer™ HCRTR1-NFAT-bla CHO-K1 cells contain the human Hypocretin (Orexin) Receptor 1 (HCRTR1), (Accession No. NM_001525) stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat. No. K1078) contain a beta-lactamase (bla) reporter gene under control of the NFAT Response Element.
The HCRTR1-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations of Orexin A. In addition, HCRTR1-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section.
Specifications
| BC074796.2 | |
| Dividing Cells | |
| GeneBLAzer™ HCRTR1-NFAT-bla CHO-K1 Cells, 2 x 106 Cells, ∽2,000,000 cells/vial at 2,000,000 cells/mL Quantity of Cells, CHO-K1 Cell Line, Fluorescent Detection Method, GPCRs Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage | |
| Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays | |
| GeneBLAzer™ HCRTR1-NFAT-bla CHO-K1 Cells (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol). Includes: GeneBLAzer™ HCRTR1 -NFAT-bla CHO-K1 cells, GeneBLAzer ™ HCRTR1 | |
| GeneBLAzer™ | |
| BLA (Beta-Lactamase) | |
| HCRTR1 | |
| CHO-K1 | |
| GPCR |
| Cell-based beta lactamase reporter gene | |
| LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol. Includes:• GeneBLAzer ™ HCRTR1 -NFAT-bla CHO-K1 cells • GeneBLAzer ™ HCRTR1 -NFAT-bla CHO-K1 Assay Protocol • Certificate of Analysis The GeneBLAzer™ HCRTR1 -NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt. |
|
| Fluorescence | |
| HCRTR1 | |
| Pool of Stable Cells | |
| 2 x 106 Cells | |
| CHO-K1 | |
| CHO-K1 Cells | |
| GeneBLAzer™ |
For Research Use Only. Not for use in diagnostic procedures.