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Thermo Scientific™ GeneBLAzer™ MR-UAS-bla HEK 293T Cells
MR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Mineralcorticoid Receptor (MR) fused to the NA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.
Brand: Thermo Scientific™ K1696
This item is not returnable.
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Description
MR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Mineralcorticoid Receptor (MR) fused to the NA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-MR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384- well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. MR-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of aldosterone. In addition, MR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and substrate loading time. Additional testing data using alternate stimuli are also available.
Specifications
| AM 580, Fenretinide, 9-cis- retinoic acid, Ch 55, TTNPB | |
| Cell-based beta lactamase reporter gene | |
| MR-UAS-bla HEK 293T cells (K1696) Shipping: Dry Ice Storage: -80°C (liquid nitrogen), Liquid Nitrogen |
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| GeneBLAzer™ MR-UAS-bla HEK 293T Cells, 2 x 106 Cells, LE 135 Antagonists, Fluorescent Detection Method, Nuclear Receptors Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage | |
| Nuclear Receptors | |
| 2 x 106 Cells | |
| GeneBLAzer™ | |
| HEK 293T Cells | |
| BLA (Beta-Lactamase) | |
| Cell Line |
| LE 135 | |
| Dividing Cells | |
| Fluorescence, Fluorescent | |
| Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays | |
| GeneBLAzer™ MR-UAS-bla HEK 293T cells Includes: MR-UAS-bla HEK 293T cells, Protocol, Certificate of Analysis | |
| All-trans-retinoic Acid | |
| 2 x 106 Cells | |
| 293T (HEK) | |
| NR3C2, MR |
For Research Use Only. Not for use in diagnostic procedures.