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Invitrogen™ GeneBLAzer™ PPAR alpha UAS-bla HEK 293T Cellular Assay Kit

GeneBLAzer™ PPAR alpha UAS-bla HEK 293T Cellular Assay Kit

Brand:  Invitrogen™ K1875

Additional Details : Weight : 0.04000kg

Product Code. 10467888

  • 1110.00 € / Each
Estimated Shipment: 23-07-2024
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Description

Description

The GeneBLAzer™ PPAR alpha-UAS-bla HEK 293T Cellular Assay provides an accurate, sensitive, and easy-to-use method for monitoring the cellular response of PPAR alpha to drug candidates or other stimuli.

Features of the The GeneBLAzer™ PPAR alpha-UAS-bla HEK 293T Cellular Assay:
  • Convenience—plate cells and perform assay without additional cell culture steps
  • Low Noise—the kit uses a Fluorescence Resonance Energy Transfer (FRET)-based ratiometric readout for minimum noise and error
  • Low Background—the GAL4 fusion prevents any background from endogenous receptor

    The Cells
    The GeneBLAzer™ PPAR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human peroxisome proliferator-activated receptor alpha (PPAR alpha) fused to the DNA-binding domain of GAL4 transiently transduced (via BacMam virus) into the GeneBLAzer™ UAS-bla HEK293T cell line. GeneBLAzer™ UAS-bla HEK 293T cells stably express a β-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS).

    The Assay
    When an agonist binds to the LBD of the GAL4 (DBD)-PPAR alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of β-lactamase. The FRET ratiometric readout from the live-cell substrate reduces the absolute and relative errors that can mask the underlying biological response of interest. PPAR alpha UAS-bla HEK 293T cells have been tested for assay performance using variable assay conditions, including cell number, stimulation time, substrate loading time and have been validated for Z' and EC50 concentrations of GW7647. Additional testing data using alternate stimuli are also available. See the 'validation packet' in the documents below to view the data.

    Speed and Convenience
    Because PPAR alpha UAS-bla HEK 293T cells are transiently transduced with BacMam virus, they are ready to be used in an assay without any additional cell culture steps. Just plate the cells and assay as outlined in the protocol.

    Please email our Custom Services team to inquire about obtaining these cells at economical bulk scales.
    • Specifications

    Specifications

    Liquid Nitrogen
    Fluorescent
    GeneBLAzer™ PPAR alpha UAS-bla HEK293T Assay Kit Each system contains sufficient transduced cells and substrate to assay one 384-well plate. Includes: PPAR alpha UAS-bla HEK293T cells, LiveBLAzer™-FRET B/G Loading Kit, 70μg
    293T (HEK)
    GeneBLAzer™ PPAR alpha UAS-bla HEK 293T Cellular Assay Kit, 1 Plate, Fluorescent Detection Method, Nuclear Receptors Druggable Target, BLA (Beta-Lactamase) Reporter, Liquid Nitrogen Storage
    Pharma and biopharma, drug discovery and development, target and lead identification and validation, G-protein coupled receptor biology, cell-based GPCR reporter assays
    1 Plate
    Activity Detection Assay Kit
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    Special Offers

    Special Offers

    For Research Use Only. Not for use in diagnostic procedures.