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Thermo Scientific™ GeneBLAzer™ PPARγ-UAS-bla HEK 293H Cells
PPAR gamma-UAS-bla HEK 293H cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-gamma (PPAR gamma) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293H cell line.
Brand: Thermo Scientific™ K1701
This item is not returnable.
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Description
PPAR gamma-UAS-bla HEK 293H cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-gamma (PPAR gamma) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293H cell line. GeneBLAzer™UAS-bla HEK 293H cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-PPAR gamma (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. PPAR gamma-UAS-bla HEK 293H cells are functionally validated for Z' and EC50 concentrations of Rosiglitazone. In addition, PPAR gamma-UAS-bla HEK 293H cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.
Specifications
| Cell-based beta lactamase reporter gene | |
| PPAR gamma-UAS-bla 293H cells (K1094) Shipping: Dry Ice Storage: -80°C (liquid nitrogen), Liquid Nitrogen |
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| Nuclear Receptors | |
| Nuclear Receptors | |
| GeneBLAzer™ | |
| HEK 293H Cells | |
| PPARG, PPAR gamma, NR1C3 |
| Dividing Cells | |
| Fluorescence, Fluorescent | |
| Human | |
| Calcitriol | |
| 2 x 106 Cells | |
| BLA (Beta-Lactamase) | |
| Dividing Cells |
For Research Use Only. Not for use in diagnostic procedures.