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Thermo Scientific™ GeneBLAzer™ PR DA Assay Kit
GeneBLAzer™PR DA (Division Arrested) cells and PR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Progesterone Receptor (PR) fused to the DNAbinding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.
Brand: Thermo Scientific™ K1403
This item is not returnable.
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Description
GeneBLAzer™PR DA (Division Arrested) cells and PR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Progesterone Receptor (PR) fused to the DNAbinding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-PR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384- well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both PR DA cells and PR UAS- bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of R5020. In addition, PR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
Specifications
| GW 3965, 24 (S), 25-Epoxycholesterol, 22 (R) OH-Cholesterol, 24 (S) OH-Cholesterol | |
| Division-Arrested Cells | |
| Fluorescence | |
| 5241 | |
| TO901317 | |
| 1 plate | |
| PGR, NR3C3, PR | |
| 293T (HEK) | |
| Nuclear Receptors |
| Cell-based beta lactamase reporter gene | |
| Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate: • PR HEK 293T DA cells (K1403A) • LiveBLAzer™-FRET B/G Loading Kit, 70 μg Shipping: Dry Ice Storage: -80°C (liquid nitrogen) |
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| Nuclear Receptors | |
| PR | |
| GeneBLAzer™ | |
| BLA (Beta-Lactamase) | |
| Division-Arrested Cells Assay Kit | |
| GeneBLAzer™ |
For Research Use Only. Not for use in diagnostic procedures.