Genomic DNA isolation, Lysis Buffer T1, Macherey-Nagel™

Description

NucleoSpin™ Tissue is designed for the rapid purification of highly pure genomic DNA from tissue samples, mouse tails, bacteria, yeast, forensic samples and clinical samples. Up to 35μg of high purity genomic DNA can be prepared (typical yields from tissue or cells: 15 to 25μg). The obtained DNA can be used directly for PCR*, Southern blotting or any kind of enzymatic reaction. With the NucleoSpin™ Tissue method, lysis is achieved by incubation of the samples in a solution containing SDS and proteinase K at 56°C. Appropriate conditions for binding of DNA to the silica membrane of the NucleoSpin™ Tissue columns are created by addition of large amounts of chaotropic ions (buffer B3) plus ethanol to the lysate. The binding process is reversible and specific for nucleic acids. Contaminations are removed by efficient washing. Pure genomic DNA is finally eluted under low ionic strength conditions in a slightly alkaline elution buffer and is ready to use for subsequent reactions. Kit components: NucleoSpin™ Tissue columns, 2mL collecting tubes, buffers, proteinase K. For further information on this product please contact Customer Services.

• Silica membrane technology
• Sample size: up to 25mg to tissue or 10⁷ cells
• Yield: up to 35μg genomic DNA
• Elution volume: 60μL to 100μL
• Binding capacity: 60μg
• Preparation time: 20min/4 to 6 preps
• Format: mini spin columns
• Spin columns can be closed – no cross-contamination
• No use of organic solvents
• Highly pure nucleic acids suitable for all common downstream applications Total DNA extraction possible from: Tissue (e.g., mouse tails); Cells (e.g., bacteria); Clinical samples (stool, urine, biopsy samples); Forensic samples (dried blood spots, hair, buccal swabs, cigarette filters).