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Gibco™ Medium 199, Earle's Salts, powder
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.
Brand: Gibco™ 31100019
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Description
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This M199 is modified as follows:
With: L-glutamine, Phenol Red
Without: HEPES, Sodium Bicarbonate
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Using Medium 199
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
Order Info
Shipping Condition: Room Temperature
Specifications
| Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture |
|
| Chick Embryo Fibroblasts, Mouse Pancreatic Epithelium, Rat Lens Tissues | |
| Low Glucose, Phenol Red | |
| cGMP-compliant under the ISO 13485 standard | |
| 50 L |
| Rat epithelial | |
| Powder | |
| No HEPES, No Sodium Pyruvate | |
| Gibco™ | |
| Room Temperature |