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Invitrogen™ BaculoDirect™ C-Term Transfection Kit
Powerful and versatile eukaryotic system for high-level protein expression in insect cells
Brand: Invitrogen™ 12562039
1038.60 EUR valid until 2025-03-28
Use promo code "24107" to get your promotional price.
Includes: Five 0.3μg vials of BaculoDirect Linear DNA, 125μL of Cellfectin transfection reagent, and ganciclovir.
Description
- Engineered to include attR sites for quick and efficient recombination with Gateway entry clone
- Gene of interest is recombined from entry clone into BaculoDirect Linear DNA using simple, one-hour LR reaction
- Resulting reaction mix contains recombinant baculovirus carrying gene of interest and is used directly to transfect insect cells
- Eliminates need for transforming bacteria and isolating large bacmid or co-transfection of transfer vector and linear baculovirus DNA into insect cells togreatly reduce hands-on time
- Purified baculovirus can be isolated in less than one week
- Gateway Linear DNA: Designed for rapid cloning with Gateway entry clone and subsequent expression in Sf9 or Sf21 insect cells
- Strong polyhedrin promoter for high-level expression
- R sites for efficient recombination with attL-flanked Gateway entry vector
- TK gene for negative selection using ganciclovir
- N-terminal V5-His tag for detection with Invitrogens Anti-V5 Antibody and purification with nickel-chelating resin (BaculoDirect N-Term Linear DNA)
- TEV protease cleavage site for removal of V5-His tag following purification (BaculoDirect N-Term Linear DNA)
- C-terminal V5-His tag for detection with Invitrogens Anti-V5 Antibody and purification with nickel-chelating resin (BaculoDirect C-Term Linear DNA)
Baculovirus Production and Titering, Cloning, Gateway Cloning, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
Specifications
| Polyhedrin | |
| The BaculoDirect™ transfection kits include five 0.3 μg vials of BaculoDirect Linear DNA, 125 μL Cellfectin transfection reagent, and ganciclovir. Store the BaculoDirect Linear DNA and Cellfectin at 4°C. All components are guaranteed stable for 6 months if stored properly. |
|
| Gateway | |
| BaculoDirect Vectors |
| Transfection Kit | |
| His Tag (6x), V5 Epitope Tag | |
| 5 transfections | |
| BaculoDirect™, Gateway™ |
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Product Title
Invitrogen™ BaculoDirect™ C-Term Transfection Kit > 5 transfections
For Research Use Only. Not for use in diagnostic procedures.
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