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Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli

Delivers fast, efficient and reliable cloning into various destination vectors for multitude of applications

Brand:  Invitrogen™ K252020

580.22 EUR valid until 2025-04-18
Use promo code "24107" to get your promotional price.

Product Code. 10286662

  • 894.00 € / Each

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Includes: TOPO-adapted, ready-to-use pCR8/GW/TOPO Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, Mach1-T1R Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid.
This item is not returnable. View return policy

Description

Description

  • Fast, 5-minute TOPO TA Cloning delivers 95% recombinants
  • 3'-T overhangs simplify cloning of Taq-amplified PCR products
  • Fast growing MachI cells shorten cloning time
  • Sequencing primer sites located close to cloning insertion site so sequences more insert, less vector first time
  • Spectinomycin resistance gene for robust selection in E. coli
  • EcoR I sites flanking PCR product insertion site for easy excision of inserts

Cloning,Gateway Cloning,PCR Cloning

TRUSTED_SUSTAINABILITY
Specifications

Specifications

TOPO™ with Gateway™
20 reactions
TA Cloning Kit
pCR
The pCR™8/GW/TOPO™ TA Cloning Kit contains the TOPO™-adapted, ready-to-use pCR™8/GW/TOPO™ Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, One Shot™ Mach1™-T1R Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored
One Shot™
20 Reactions
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Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli > 20 reactions

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