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Invitrogen™ pENTR™/SD/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli
Use highly efficient, 5-minute cloning strategy to directionally clone a blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System
Brand: Invitrogen™ K242020
600.60 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.
Includes: pENTR/SD/D-TOPO vector, dNTPs, salt solution, sterile water, universal M13 sequencing primers, OneShot TOP10 Chemically E. coli, S.O.C. Medium, and a pUC19 control plasmid.
Description
- Blunt-end PCR products clone directionally into pENTR/SD/D-TOPO entry vector at greater than 90% efficiency, and include an upstream Shine-Dalgarno sequence for expression in prokaryotes
- Rapidly shuttle cloned genes between multiple vector systems
- Reliable performance
- Directional cloning vector for entry to Gateway System and optimized for prokaryotic expression
- For access to Gateway System, PCR amplify the gene of interest and add product straight to the provided topoisomerase charged pENTR/SD/D-TOPO vector, incubate 5 minutes, and transform the provided competent E. coli cells
- Resulting attL containing Gateway Entry clones are ready for efficient recombination with choice of Gateway Destination vectors
- pENTR/SD/D-TOPO vectorincludes T7 gene 10 translational enhancer and ribosome binding site (RBS) for optimal expression of native protein after recombination with prokaryotic Gateway destination vectors
- Can also use vector for expression in other hosts systems by recombination with different destination vectors
- Vector has M13 and T7 primer sequencing sites and attL recombination sites flanking PCR product insertion site, allowing clones to be easily sequence-verified and recombined into choice of attR containing Gateway destination vectors
- Kanamycin resistance gene and pUC origin are used for selection and high-copy propagation in E. coli
- Four base overhang on vector pairs with four base sequence designed into forward primer used in PCR reaction to provide directionality to the topoisomerase ligation reaction
Cloning, Gateway Cloning
Specifications
| Directional TOPO™ | |
| 20 reactions | |
| TOPO Cloning Kit |
| pENTR™⁄SD⁄D-TOPO™ Cloning Kit contains pENTR⁄SD⁄D-TOPO™ vector, dNTPs, salt solution, sterile water, universal M13 sequencing primers, OneShot™ TOP10 Chemically E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store Competent E. coli at -80°C. Store all other components at -20°C. | |
| One Shot™ | |
| pENTR |
For Research Use Only. Not for use in diagnostic procedures.