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Invitrogen™ Mouse Cot-1 DNA
Mouse DNA predominantly 50 to 300 bp in size and enriched for repetitive DNA sequences such as the B1, B2, and L1 family members
Brand: Invitrogen™ 18440016
Includes: Mouse Cot-1 DNA™ is supplied at 1mg/mL in 10mM Tris-HCl (pH 7.4), 1mM EDTA.
Description
Mouse Cot-1 DNA™ is commonly used to block non-specific hybridization in microarray screening. It can also be used to suppress hybridization of rodent repetitive DNA sequences when mapping rodent clones by in situ hybridization or Southern blotting and to identify mouse clones in screens of somatic-cell hybrid libraries derived from mouse-hamster hybrid cells (1-3). The amount supplied is sufficient for 5 to 10 Southerns or 500 in situ hybridizations.
Performance and Quality Testing: Purity and DNA size are verified by agarose gel electrophoresis. Concentration is verified spectrophotometrically by diluting Mouse Cot-1 DNA™ 1:100 in 50mM NaOH and using the conversion factor 0.033μg/μL/A 260 . Other methods used to determine concentration may yield varying results.
Array CGH, Cell Analysis, Cellular Imaging, Fluorescence In Situ Hybridization, Gene Expression Analysis and Genotyping, Genotyping and Genomic Profiling, In Situ Hybridization (ISH), Microarray Analysis
Order Info
Shipping Condition: Approved for shipment on Wet or Dry Ice
Specifications
| Mouse DNA | |
| Cot-1 DNA | |
| Mouse | |
| Block Non-Specific Hybridization | |
| Liquid |
| Mouse Cot-1 DNA™ is supplied at 1 mg/ml in 10 mM Tris-HCl (pH 7.4), 1 mM EDTA. Store at -20°C. | |
| Approved for shipment on Wet or Dry Ice | |
| Cot-1 DNA™ | |
| 500 μg |
For Research Use Only. Not for use in diagnostic procedures.