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Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase

The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.

405.00 € - 1104.00 €

Specifications

Detection Method Gel Electrophoresis
Format One-Step RT-PCR System
GC-Rich PCR Performance High
PCR Method 1-step RT-PCR
Polymerase Platinum Taq High Fidelity
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Products 2
Product Code Brand No. of Reactions Price Quantity & Availability  
Product Code Brand No. of Reactions Price Quantity & Availability  
10092922
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Invitrogen™
12574030
25 Reactions
405.00 €
25 reactions
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10779764
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Invitrogen™
12574035
100 Reactions
1104.00 €
100 reactions
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Description

Description

The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR. This one-step formulation allows for both cDNA synthesis and PCR amplification to be performed in a single tube using gene-specific primers and target RNAs from either total RNA or mRNA. It also allows for the detection of a wide range of RNA targets (up to 10 kb in length) at variable concentrations (1 pg to 1 μg of total RNA). This system consists of two major components: SuperScript III RT/Platinum Taq High Fidelity enzyme mix and 2X reaction mix.

SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA and can be used to synthesize cDNA from total RNA. Platinum Taq DNA Polymerase High Fidelity is a an enzyme mixture composed of recombinant Taq DNA polymerase, Pyrococcus species GB–D polymerase, and Platinum Taq antibodies, which block polymerase activity at ambient temperatures enabling hot start PCR.

The 2X reaction mix consists of a proprietary buffer system optimized for reverse transcription and PCR amplification, Mg2+ optimized for universal use, deoxyribonucleotide triphosphates, and stabilizers.

The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity offers:
• High-fidelity end-point detection and analysis of RNA molecules by RT-PCR
• PCR amplification and cDNA synthesis in a single tube
• Detection of a wide range of RNA targets from 300 bp to 10 kb
• Increased specificity with wide cDNA synthesis temperature range of 45−60°C

Note: For superior one-step RT-PCR performance, we recommend the SuperScript IV One-Step RT-PCR System (Cat. No. 12594025) or SuperScript IV One-Step RT-PCR System with ezDNase (Cat. No. 12595025). The SuperScript IV One-Step RT-PCR System combines the high processivity of SuperScript IV Reverse Transcriptase with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched product yields, specificity, and sensitivity in less time and for a broad target range, even with suboptimally pure RNA samples.

Order Info

Shipping Condition: Dry ice

Specifications

Specifications

Gel Electrophoresis
High
Platinum Taq High Fidelity
1-Step RT-PCR
SuperScript III
Dry Ice
Built-In Hot Start
Reverse Transcription
RNA
One-Step RT-PCR System
1-step RT-PCR
30 min.
50°C
Reduced
PCR Amplified cDNA
Premixed Components
Up to 10 kb
Videos
SDS
Documents

Documents

Certificates

For Research Use Only. Not for use in diagnostic procedures.