missing translation for 'onlineSavingsMsg'
Learn More
Learn More
Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ INVαF' Chemically Competent E. coli
Quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector
Brand: Invitrogen™ K200001
385.44 EUR valid until 2024-12-20
Use promo code "21651" to get your promotional price.
This item is not returnable.
View return policy
Description
The TA Cloning Kit uses the pCR2.1 cloning vector and ExpressLink T4 DNA Ligase to generate a ligation product in a 15-minute, room-temperature ligation step. Reactions typically yield >80% recombinants containing inserts.
- Fast and convenient: 15-minute, room-temperature ligation
- Efficient: blue/white screening and >80% clones with correct insert
- Flexible: choice of kanamycin or ampicillin resistance for flexible antibiotic selection
- Hassle-free: eliminates any enzymatic modifications of PCR product
- Streamlined: does not require use of PCR primers that contain restriction sites
- 3'-T overhangs for direct ligation of Taq-amplified PCR products
- T7 promoter for in vitro RNA transcription and sequencing
- Versatile polylinker with flanking EcoR I sites for easy excision of inserts
- M13 forward and reverse primer sites for sequencing
- Taq polymerase has non-template-dependent activity that adds single deoxyadenosine (A) to the 3' ends of PCR products
- Linearized vector supplied in has single 3' deoxythymidine (T) residues
- Allows PCR inserts to ligate efficiently with vector
Cloning, PCR Cloning
Specifications
| INVαF ́ | |
| TA Cloning | |
| Kit | |
| 20 Reactions | |
| Cloning Kit | |
| pCRII |
| Chemically Competent E. coli | |
| TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
|
| PCR Cloning | |
| One Shot™ | |
| T7 |
For Research Use Only. Not for use in diagnostic procedures.