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Invitrogen™ X-Gal

X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays.

109.00 € - 368.00 €

Specifications

Detection Method Colorimetric
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
11538746
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Invitrogen™
15520034
100 mg
109.00 €
100mg
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10554973
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Invitrogen™
15520018
1 g
368.00 €
1g
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Description

Description

X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays. Bacteria containing active β-galactosidase produce blue colonies when grown on plates containing X-Gal. X-Gal is supplied as white crystals and is soluble in dimethylformamide.

Primary applications

  • Blue-white screening—detect functional β-galactosidase (reporter gene expression)
  • Histochemical detection of β-galactosidase activity in fixed tissues
  • X-gal overlay assay

Find the media format that fits your needs

We offer many ready-to-use media formulations, such as LB Broth (10855001), Terrific Broth (A1374301) or S.O.C recovery medium (15544034).

We also offer IPTG (15529019) for blue-white screening.

Explore other media formats for bacterial growth.

Order Info

Approved for shipment at Room Temperature or on Wet Ice.

Specifications

Specifications

Colorimetric
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Product Certifications

For Research Use Only. Not for use in diagnostic procedures.