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Alexa Fluor™ Kinase Tracer 199, 25 μM
Alexa Fluor™ Kinase Tracer 199 is detected by addition of a Eu-labeled anti-tag antibody.
Brand: Alexa Fluor™ PV5830
Additional Details : Weight : 0.10000kg
Description
- Color: Far-Red
- Excitation/Emission (nm): 340/665
- Label or Dye: Alexa Fluor™ 647
- Target Enzyme: Kinases (Multiple)
- Validated Kinases: EGFR T790M L858R, EPHB4, KIT V654A, MAPK9 (JNK2), RIPK2
- In the LanthaScreen™ Eu Kinase Binding Assay, binding of an Alexa Fluor™ conjugate or tracer such as Kinase Tracer 199 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps.
Specifications
| Kinase Binding Assay | |
| Far-Red | |
| 340/665 | |
| Alexa Fluor™ 647 | |
| MAPK8, EGFR, RIPK2, MAPK9, EGFR T790M L858R, MAPK14, MAPK10, EGFR L861Q, EGFR L858R | |
| EGFR T790M L858R, EPHB4, KIT V654A, MAPK9 (JNK2), RIPK2 |
| Biochemical binding/kinetic | |
| Store at -20°C. | |
| TR-FRET | |
| Dry Ice | |
| Kinase Tracer |
For Research Use Only. Not for use in diagnostic procedures.