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Thermo Scientific™ LanthaScreen™ TR-FRET VDR Coactivator Assay Kit, goat
Contains Goat Tb-Anti-GST antibody
Brand: Thermo Scientific™ PV4554
This item is not returnable.
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Description
- This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15127
- The LanthaScreen™ TR-FRET Vitamin D Receptor (VDR) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential VDR ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a VDR ligand-binding domain (VDR-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-and-read assay format
- To run the LanthaScreen™ TR-FRET VDR Coactivator Assay, VDR-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti-GST antibody. After room temperature incubation, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound.
- Based upon the biology of the VDRcoactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).
- The LanthaScreen™ TR-FRET VDR Coactivator Assay Kit contains the VDR-LBD (GST) protein, fluorescein-labeled TRAP220/DRIP-2 coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
Specifications
| Tb (Terbium) | |
| Microplate Reader | |
| Store in ultra-cold freezer (-68 to -85°C). | |
| 7421 | |
| Dry Ice | |
| LanthaScreen™ | |
| VDR | |
| 800 x 20 μL Assays |
| Fluorescence | |
| 384-well Plate | |
| Biochemical coregulator interaction | |
| End Point | |
| VDR, NR1I1 | |
| Co-Factor Interaction Assay, TR-FRET | |
| TR-FRET VDR Coactivator Assay Kit |
For Research Use Only. Not for use in diagnostic procedures.