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Invitrogen™ Mouse LIX/CXCL5 ELISA Kit
ELISA
Brand: Invitrogen™ EMCXCL5X5
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Description
The Mouse LIX (CXCL5) ELISA quantitates Ms LIX in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms LIX. Principle of the method The Mouse LIX solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
The protein encoded by this gene is an inflammatory chemokine that belongs to the CXC chemokine family. This chemokine is produced concomitantly with interleukin-8 (IL8) in response to stimulation with either interleukin-1 (IL1) or tumor necrosis factor-alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.
Specifications
| P50228 | |
| 35 pg/mL | |
| Solid-phase sandwich ELISA | |
| ELISA | |
| Plasma, Serum, Supernatant | |
| 5 x 96 assays | |
| ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
| 20311 | |
| <12% | |
| Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| 5 x 96 Tests | |
| Inflammation | |
| 2°C to 8°C | |
| CXCL5 | |
| 4 hrs 45 mins |
| 31.25 to 2000 pg/mL | |
| 35 pg/mL to 2000 pg/mL | |
| HRP | |
| Chemokine & Receptors | |
| This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), L-Selectin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF. | |
| Mouse | |
| Colorimetric Microplate Reader | |
| AMCF-II,Cxcl6,ENA-78,GCP-2,LIX,Scyb5,Scyb6 | |
| <10 | |
| HRP | |
| RUO | |
| Plasma 20 μL, Serum 20 μL, Supernatant 50 μL | |
| AMCF-II, ENA-78, GCP-2, LIX, Scyb5, Scyb6 | |
| 1 hrs 20 mins |
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