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Invitrogen™ LIX (CXCL5) Rat ELISA Kit
Sandwich ELISA Kit
Brand: Invitrogen™ ERCXCL5
Includes: Pre-coated plate(s), standard, detector antibody, HRP conjugate, diluents, wash buffer, chromogen, stop solution, and plate covers.
Description
The Rat LIX (CXCL5) ELISA quantitates Rt LIX in rat serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Rt LIX. Principle of the method The Rat LIX solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
CXCL6 is a chemotactic for neutrophil granulocytes. It signals through binding and activation of its receptors (CXCR1 and CXCR2). In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (90-fold-higher when compared to CXCL5 and CXCL7).
Specifications
| P97885 | |
| 20 pg/mL | |
| Solid-phase sandwich ELISA | |
| ELISA Kit | |
| Plasma, Serum, Supernatant | |
| 96 assays | |
| ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
| 60665 | |
| <12% | |
| Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| 96 Tests | |
| Immunology | |
| 2°C to 8°C | |
| Rat | |
| 4 hr. 45 min. |
| 24.69-6000 pg/mL | |
| 20pg/mL to 6000pg/mL | |
| Biotin | |
| Chemokine & Receptors | |
| This ELISA kit shows no cross-reactivity with any of the cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, MCP-1, MIP-3 alpha, beta-NGF, TIMP-1, TNF-alpha, VEGF. | |
| Rat | |
| Colorimetric Microplate Reader | |
| Cxcl5, Cxcl6 | |
| <10% | |
| HRP | |
| RUO | |
| Plasma, 33 μL; Serum, 33 μL; Supernatant, 100 μL | |
| Cxcl5 | |
| 1 hr. 20 min. |
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