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Gibco™ Medium 199, Earle's Salts (10X)
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.
Brand: Gibco™ 21180021
29.20 EUR valid until 2024-11-29
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This item is not returnable.
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Description
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This Medium 199 is modified as follows:
With: Phenol Red, L-glutamine
Without: HEPES, Sodium Bicarbonate
The complete formulation is available.
Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Concentrated forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
Specifications
| Storage conditions: 2-8° C. Protect from light. Shipping conditions: Ambient. Shelf life: 12 months from date of manufacture. |
|
| Mammalian Cells | |
| 10 X | |
| Liquid | |
| Sterile-filtered | |
| No HEPES, No Sodium Bicarbonate | |
| cGMP-compliant under the ISO 13485 standard | |
| 2 to 2.8 | |
| 500 mL |
| CO2 | |
| Animal Origin | |
| Mammalian Cell Culture | |
| Medium 199 | |
| Glutamine, Phenol Red, Earle's Balanced Salt Solution (EBSS), Sodium Pyruvate | |
| Sustainable packaging | |
| 2400 - 2900 mOsm/kg | |
| Gibco™ | |
| Room Temperature |
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.