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Gibco™ Medium 199, powder
Medium 199, powder
Brand: Gibco™ 31100027
This item is not returnable.
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Description
Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco™ Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco™ Medium 199 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ Medium 199 product made in our Grand Island facility (31100-035). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment and filtration at the time of preparation (see protocol for details).
Specifications
ISO 13485 standards, FDA | |
Medium 199, 1L, Powdered Form, With Phenol Red, L-glutamine, Sodium Pyruvate, 4.1 to 4.7 pH, 36 Months Shelf Life, 240 to 300mOsm/kg Osmolality, 2 to 8°C Storage Conditions, Used for Mammalian Cell Culture | |
Medium 199 | |
No HEPES, No Sodium Bicarbonate | |
4.1 to 4.7 | |
For research use only |
2 to 8°C | |
Powder | |
Phenol Red, Sodium Pyruvate | |
Mammalian cell culture | |
1 L |