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Gibco™ MEM α, nucleosides, GlutaMAX™ Supplement
MEM α, nucleosides, GlutaMAX™ Supplement
Brand: Gibco™ 32571028
This item is not returnable.
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Description
Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco™ MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.Gibco™ MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. Gibco™ MEM α with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. This product is made with Earle’s salts.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco™ MEM α is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ MEM α product made in our Grand Island facility. This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Specifications
ISO 13485 standards, FDA | |
Minimum Essential Medium α±, 500mL, Liquid, 1 X Concentration, 7.0 to 7.4 pH, With Ribonucleosides, Deoxyribonucleosides, Glutamax™, Phenol Red, Sodium Pyruvate, 12 Months Shelf Life, 280 to 330mOsm/kg Osmolality, For Mammalian Cell Culture | |
Liquid | |
GlutaMAX, Deoxyribonucleosides, Sodium Pyruvate | |
7.0 to 7.4 |
2 to 8°C | |
1 X | |
Minimum Essential Medium α± | |
Mammalian cell culture | |
500 mL |
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