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Gibco™ MEM α, nucleosides, powder
Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells.
Brand: Gibco™ 11900016
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Description
Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco™ MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM α is modified as follows:
With: Ribonucleosides, Deoxyribonucleosides, L-glutamine, Phenol Red
Without: Sodium Bicarbonate
The complete formulation is available.
Gibco™ MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle’s salts.
MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
Specifications
| Storage conditions: 2-8° C. Shipping conditions: Ambient. Shelf life: 36 months from date of manufacture. |
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| Mammalian Cells | |
| Mammalian Cell Culture | |
| Minimum Essential Medium (MEM) alpha | |
| Glutamine, Phenol Red, Sodium Pyruvate | |
| cGMP-compliant under the ISO 13485 standard | |
| 3.3 to 3.9 | |
| 1 L |
| CO2 | |
| Animal Origin-free | |
| Powder | |
| Standard Serum Supplementation | |
| No HEPES, No Sodium Bicarbonate | |
| 240 - 290 mOsm/kg | |
| Gibco™ | |
| Room Temperature |