Invitrogen™ ActinRed™ 555 ReadyProbes™ Reagent (Rhodamine phalloidin)

Description

• Formulated as room temperature-stable solution and comes in convenient dropper bottle: tip and drip2 drops/mL to stain cells
• High-affinity staining of F-actin with superior specificity compared to antibody methods
• Outstanding fluorescence performance (Ex/Em: 540/565nm)
• Ready-to-use liquid formulation: no need to dilute, weigh, or pipet
• Phalloidin is a bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin
• Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low nonspecific binding
• Phalloidin binds F-actin with high selectivity, while TRITC provides red-orange fluorescence of unparalleled brightness and photostability
• Demonstrating very little nonspecific staining, allows high-contrast discrimination of actin staining
• Visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations
• Fully compatible with other fluorescent stains used in fixed-cell analyses, including antibodies, fixed-cell dyes, fluorescent proteins, and Qdot probe
• Validated and robust reagent for studying cytoskeleton in the context of stem cell differentiation, cancer biology, as marker in RNAi library screens, or target in drug discovery
• May be added directly to fixed cells in full media or buffer solutions
• Optimization may be needed for some cell types, conditions, and applications: In such cases, simply add more or fewer drops until the optimal staining intensity is obtained
• Detected through standard TRITC (Rhodamine/DiI/Cy3/Alexa Fluor 546) filters

Cell Analysis, Cell Structure, Cytoskeleton