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Invitrogen™ EnzChek™ Phosphatase Assay Kit
Continuous assay of prostatic acid phosphatase and protein phosphatase 1
Brand: Invitrogen™ E12020
This item is not returnable.
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Description
Using the EnzChek Phosphatase Assay Kit continuous monitoring of phosphatase activity, even at neutral or acidic pH, can be achieved. The kit contains Molecular Probes (proprietary) 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) substrate. DiFMUP is about 100 times more sensitive than 4-methylumbelliferyl phosphate (MUP) for the detection of prostatic acid phosphatase at pH 5.5. The kit is perfect for the continuous assay of prostatic acid phosphatase, protein phosphatase 1 or almost any other phosphatase that can be assayed with nonprotein-based substrates such as MUP or 4-nitrophenyl phosphate (PNPP). Each kit contains a sufficient amount of substrate for ∽1000 assays, using a reaction volume of 100μL per assay. Fluorescence (excitation/emission maxima approx. 358/455nm) can be measured in a fluorescence microplate reader or a standard fluorometer.
Cell Analysis, Cellular Imaging, Drug Discovery and Development, Enzyme and Protein Activity Assays, High-Content Screening (HCS), Phosphatase Activity, Phosphatase Activity Assays, Phosphatase Biology, Proteins, Expression, Isolation and Analysis, Target and Lead Identification and Validation
Order Info
Shipping Condition: Room temperature
Specifications
| Blue | |
| Fluorescence | |
| Microplate Reader | |
| DiFMU | |
| EnzChek™ | |
| Room Temperature | |
| Chemical Substrate | |
| Phosphatases |
| Store in freezer -5°C to -30°C and protect from light. | |
| Phosphatase Assay | |
| Phosphatase Assay | |
| 360⁄460 | |
| 1000 Assays | |
| DiFMUP | |
| Phosphatase Substrate |
Safety and Handling
Product Identifier
Signal Word
Hazard Category
Hazard Statement
Precautionary Statement
Supplemental information
- EnzChek Phosphatase Assay Kit
Signal Word
- Danger
Hazard Category
- Acute toxicity Category 4
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 3
- Reproductive toxicity Category 1B
Hazard Statement
- H226-Flammable liquid and vapour.
- H312-Harmful in contact with skin.
- H319-Causes serious eye irritation.
- H332-Harmful if inhaled.
- H360D-May damage the unborn child.
Precautionary Statement
- P201-Obtain special instructions before use.
- P202-Do not handle until all safety precautions have been read and understood.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P233-Keep container tightly closed.
- P240-Ground/bond container and receiving equipment.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P242-Use only non-sparking tools.
- P243-Take precautionary measures against static discharge.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P271-Use only outdoors or in a well-ventilated area.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P302+P352-IF ON SKIN: Wash with plenty of water/soap.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P308+P313-IF exposed or concerned: Get medical advice/attention.
- P312-Call a POISON CENTER/doctor/if you feel unwell.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P370+P378-In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
Supplemental information
- MIXTURE LIST-Contains : Dimethylformamide
For Research Use Only. Not for use in diagnostic procedures.