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  5. Invitrogen™ Mouse Myeloperoxidase ELISA Kit

Invitrogen™ Mouse Myeloperoxidase ELISA Kit

Product Code. 15485313
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Quantity:
10 x 96 Tests
5 x 96 Tests
96 Tests
Unit Size:
1 set
Each
Pack of 10
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Product Code. 15485313

Brand: Invitrogen™ EMMPO

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Description

ELISA

The Mouse Myeloperoxidase ELISA quantitates Ms Myeloperoxidase in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Myeloperoxidase. Principle of the method The Mouse Myeloperoxidase solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Myeloperoxidase (MPO) is a heme protein that is synthesized during myeloid differentiation and is a major component of neutrophil azurophilic granules. It is produced as a single chain precursor and subsequently cleaved into a light and heavy chain. The mature MPO is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme is responsible for producing hypohalous acids that are central to the microbicidal activity of neutrophils. Diseases that are associated with MPO include Myeloperoxidase Deficiency and Alzheimer's Disease, Familial, 1.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number P11247
Assay Range 0.61 to 150 ng/mL
Assay Sensitivity 600 pg/mL
Calibration Range 0.6 ng/mL to 150 ng/mL
Description Myeloperoxidase ELISA Kit, 96 Assays, 1 Kit, Mouse Species, HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Myeloperoxidase (MPO) in Samples Using a Microplate Reader
Detectable Analytes Myeloperoxidase (MPO), myeloperoxidase (MPO), mouse MPO
Assay Solid-phase sandwich ELISA
Conjugate HRP
Detection Method Colorimetric
Product Type ELISA
Protein Family Other Proteins
Sample Type Plasma, Serum, Supernatant
Specificity This ELISA antibody pair detect mouse MPO. Other species not determined yet
Sufficient For 96 Determinations
Target Species Mouse
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 17523
Gene Symbol mKIAA4033
Host Species Mouse
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Research Discipline Other Research
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
Storage Requirements 2°C to 8°C
Synonym RP23-449P23.5, mKIAA4033
Target Myeloperoxidase
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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