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Novex™ Novex™ Zymogram Plus (Gelatin) Protein Gels, 10%, 1.0 mm
Novex 10% Zymogram Plus (Gelatin) gels are useful for the detection and characterization of proteases that use gelatin as a substrate. The proteases are run under denaturing conditions and visualized as clear bands against a dark background using a simple renaturing, developing, and staining protocol.
Brand: Novex™ ZY00100BOX
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Description
Novex 10% Zymogram Plus (Gelatin) gels are useful for the detection and characterization of proteases that use gelatin as a substrate. The proteases are run under denaturing conditions and visualized as clear bands against a dark background using a simple renaturing, developing, and staining protocol. Zymogram gels are commonly used to detect matrix metalloproteinases. Novex 10% Zymogram Plus gels are highly sensitive, detecting as little as 5 x 10-6 units of collagenase.
Formulation
Novex Zymogram Plus gels are Tris-glycine gels with 0.1% gelatin incorporated into the gel. They are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, highly purified water, and gelatin as the substrate. All have a 4% stacking gel.
Choose the right gel format for your experiments
Novex 10% Zymogram Plus gels come in a fixed concentration of 10% gelatin and three different well formats, including 10-, 12- and 15-well.
Recommended buffers
Use Novex Tris-Glycine SDS Sample Buffer (LC2676) and Novex Tris-Glycine SDS Running Buffer (LC2675) with these gels. Novex Zymogram Renaturing Buffer (LC2670) and Novex Zymogram Developing Buffer (LC2671) are also recommended.
Recommended Storage
Store in refrigerator (2-8°C). Do not freeze.
Specifications
| Mini Gel Tank, XCell SureLock™ Mini-Cell | |
| Novex | |
| Zymogram Gel | |
| 10-well | |
| Up to 25 μL | |
| Wet Ice | |
| 1.0 mm |
| Mini | |
| 0.1 | |
| Molecular Weight | |
| 10 Gels/Box | |
| 30 to 150 kDa (Tris-Glycine Buffer) | |
| Store at 2°C to 8°C. Do not freeze. | |
| 8 cm |